Sex Linked Developmental Rate Differences in Murrah Buffalo (Bubalus bubalis) Embryos Fertilized and Cultured In Vitro
نویسندگان
چکیده
منابع مشابه
IN VITRO PRODUCTION OF BUFFALO (Bubalus bubalis) EMBRYOS
Two experiments (Exp) were carried out to test the effect of different media on in vitro maturation (IVM) (Exp 1; n = 1142 ovaries with 2666 oocytes) and to study in vitro fertilization (IVF) of buffalo oocytes (Exp 2; n = 110 ovaries with 330 oocytes). In exp 1 the collected cumulus oocyte complex (COCs) was allocated to three maturational media; TCM-199 supplemented with hormones (M1), TCM-19...
متن کاملSex-related differences in the developmental rate of in-vitro matured/in-vitro fertilized ovine embryos.
Sex determination of in-vitro matured/in-vitro fertilized ovine embryos cultured in synthetic oviduct fluid (SOF) medium was performed by the polymerase chain reaction amplification of specific Y DNA sequences so as to test the influence of sex on developmental growth during the preimplantation period. At 144 h post-insemination, embryos with a blastocoel were classified as the fast-developing ...
متن کاملAnalysis of In Vitro Effects of Sex Steroids on Lymphocyte Responsiveness in Murrah Buffaloes (Bubalus bubalis)
Present study was carried out on forty four apparently healthy Murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro. Estrogen inhibited proliferation in mitogen-stimulated lymphocytes from prepubertal but not post pubertal buffaloes of either sex. Estrogen at 100 pg/mL concentration stimulating the proliferation sig...
متن کاملIn vitro production of cattle-water buffalo (Bos taurus--Bubalus bubalis) hybrid embryos.
Interspecific hybrid embryos are useful models for the study of maternal-fetal interactions, transmission pattern of species-specific markers and parental contributions to growth and developmental potential of pre-attachment embryos. In an attempt to investigate the possibility of producing hybrid embryos of domestic cattle (Bos taurus) and water buffalo (Bubalus bubalis), cattle oocytes were e...
متن کاملFull-term Development of in vitro Produced-Vitrified Water Buffalo (Bubalus bubalis Linn) Embryos
Assisted reproductive technologies such as artificial insemination (AI) and multiple ovulation and embryo transfer (MOET) have been introduced to overcome reproductive inefficiencies and accelerate genetic gain. However, MOET in water buffalo is compromised by the poor ovulatory response of this animal species to hormonal treatment (Karaivanov 1986; Karaivanov et al. 1990; Misra et al. 1990; Cr...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 1999
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.1999.15